Understanding Development of Chlamydia trachomatis
Research focus was on understanding the developmental cycle and varying cell forms of Chlamydia trachomatis
Our lab utilized promoter reporter plasmid vectors with an array of molecular biology techniques
Used CRISPR interference and inducible riboswitch plasmid constructs to control gene expression in Chlamydia
Microscopy
Proficient in fluorescent live-cell microscopy and confocal microscopy as well as light microscopy
Skilled in immunohistochemistry as well as immunofluorescence.
Worked with a variety of staining techniques including antibody staining, enzyme staining, and Fluorescent in-situ hybridization
Skilled in utilizing imaging software such as Fiji, ImageJ, and Illustrator to process images
Infectious Culture
8+ years of experience working with infectious culture in a biosafety 2+ lab setting
Skilled in eukaryotic host cell culture, maintained multiple culture flasks for infectious assays
Worked with Cos-7 and Hela cells
Performed lipid-based transfections
Molecular Biology techniques
Research has emphasized molecular biology, particularly genetic engineering of plasmid vectors
Proficient in PCR, ddPCR, primer design/sequencing, electrophoresis, and vector construction
Expertise with DNA/RNA/Protein isolations, Western Blot, RNA-seq, DNA-seq
Familiar with ELISA and other antibody staining techniques